Salary for this Role:

From £31,675 per annum with benefits, subject to skills and experience

Job Title:

Reports to:

Closing Date:

Job Description:

Job title: Laboratory Research Scientist (LRS) – Ratcliffe Lab

Reporting to: Peter Ratcliffe, Clinical Director,

Contract term: This is a full-time, fixed term (2 years) position on Crick terms and conditions of employment.

Summary

We are seeking an enthusiastic and proactive Laboratory Research Scientist to join a world-leading laboratory working on the sensing and signalling of oxygen levels in cells. The work has broad relevance in biology and direct relevance to many or most human diseases, including heart and lung disease, stroke and cancer. The laboratory is ‘problem-based’ and deploys a very wide range of technologies to address these problems. Further information can be found about the group at https://www.crick.ac.uk/research/labs/peter-ratcliffe

Project summary

Although the laboratory has been successful in elucidating mechanisms underlying transcriptional responses to low oxygen (Nobel Prize in Medicine or Physiology, 2019) it is clear that other systems must exist to organise responses that are transduced locally in different parts of the cell or operate at different oxygen concentrations and over different time scales. The laboratory is investigating candidate oxygen sensing systems based on the function of specific 2-oxoglutarate dependent dioxygenases that catalyses the post-translational hydroxylation of amino acid residues including in unstructured domains of proteins involved in the formation of molecular condensates. The project aims to test the hypothesis that alterations in catalysis in hypoxic cells promote re-organisation of intracellular molecular condensates to adapt cellular functions to hypoxic stress.

The Role

The role of a Laboratory Research Scientist (LRS) in the Crick is diverse and rewarding. The role covers a wide range of different functions and allows for continuity of lab operations. The role offers support to one or more scientific projects or programmes.

The LRS applies specific technical or practical skills and has a well-developed working knowledge of technical or scientific practices and procedures acquired through on-the-job, vocational or academic training. Work will involve the application of skills and knowledge to provide a range of technical support activities.

Key responsibilities

These include but are not limited to:

• Provision of technical and research support in the area of hypoxic cell biology including the tissue culture of relevant specialised cells

• Preparation and analysis of samples for proteomic mass spectrometry (MS), including discovery and targeted MS analyses

• Contribute to the development of assays of oxygen sensitivity in cultured cells including fluorescence microscopy

• Performance of cell engineering under the direction and supervision of senior laboratory staff

• Compliance with all regulatory and safety protocols

• Accurate organisation of work and experimental record keeping

Experience and Competencies

The post holder should embody and demonstrate our core Crick values:

Bold; Open; Collegial

Essential

• BSc or MSc in biochemistry, cell biology or related discipline

• Good working knowledge of general molecular biology techniques including tissue culture

• Ability to work accurately and maintain good records

• Strong collaborative ability and teamwork experience

Desirable

• Experience in protein mass spectrometry

• Experience in genetic engineering of cells

• Experience in fluorescence microscopy

At the Crick, we conduct research at the forefront of biomedical research. We combine rigour with an open and collaborative culture, and are outward-looking, reflecting our status as a partnership of six organisations aiming to pool knowledge, ideas and resources.

We have a wide research portfolio with no divisions or departments, bringing biomedical researchers together with clinicians, physical scientists and applied scientists from our pharmaceutical partners.

We aim to attract the most talented researchers and support them to tackle innovative research questions. Our science technology platforms provide our researchers with access to state-of-the-art technology and expertise.

We provide an excellent learning environment with dedicated education programmes in public engagement with science, education and personal development, and a postdoc training programme that prepares scientists for leadership roles in science.

• If you are interested in applying for this role, please apply via our website .

• All offers of employment are subject to successful security screening and continuous eligibility to work in the United Kingdom.

Find out what benefits the Crick has to offer:

For more information on our great pay and benefits package please click here: https://www.crick.ac.uk/careers-and-study/life-at-the-crick/pay-and-benefits

Equality, Diversity & Inclusion:

We welcome applications from all backgrounds. We are committed to providing equal employment opportunities, regardless of ethnicity, nationality, gender, sexual orientation, gender identity, religion, pregnancy, age, disability, or civil partnership, marital or family status. We particularly welcome applications from people who are Minority Ethnic as they are currently underrepresented in the Crick at this level.

Diversity is essential to excellence in scientific endeavour. It increases breadth and perspective, leading to more innovation and creativity. We want the Crick to be a place where everyone feels valued and where diversity is celebrated and seen as part of the foundation for our Institute’s success.

The Crick is committed to creating equality of opportunity and promoting diversity and inclusivity. We all share in the responsibility to actively promote dignity, respect, inclusivity and equal treatment and it is our aim to ensure that these principles are reflected and implemented in all strategies, policies and practices.

Read more on our website: https://www.crick.ac.uk/careers-and-study/life-at-the-crick/equality-diversity-and-inclusion