Vanderbilt University
Research Technician, Molecular Physiology and Biophysics
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Job Description
- Req#: 10005733
- Manage, order, and maintain key lab reagents and equipment
- Perform cloning and assembly of recombinant proteins
- Execute expression and purification of recombinant proteins
- Opportunity to learn structural biology or biophysical techniques
- Opportunity to conduct independent research projects
- Bachelor’s degree in a related field (biology, chemistry, physics, or related field) is necessary.
- Master’s degree is preferred.
- Experience in molecular biology and protein expression is strongly preferred
- Experience in structural biology is preferred
The research technician will work in Dr. Stephanie Wankowicz’s lab within the Center for AI for Protein Dynamics and the Molecular Physiology and Biophysics department at Vanderbilt University. This position requires knowledge of basic laboratory procedures and experience in cloning, protein expression, and protein purification.
The research technician will support ongoing research into protein structure, dynamics, and function. Additional responsibilities include laboratory maintenance such as preparing buffers, organizing chemical storage, and managing lab inventory.
The Wankowicz lab aims to elucidate the role of entropy in substrate specificity and catalysis, striving to provide a comprehensive thermodynamic understanding of enzyme function. The lab develops innovative computational structural modeling methods combined with biophysical measurements and machine learning to explore how proteins utilize entropy for binding. This position focuses on the wet lab aspects, employing X-ray crystallography and cryo-EM alongside biophysical techniques like surface plasmon resonance (SPR) and isothermal titration calorimetry (ITC) to study how different genetic variants or ligands impact protein dynamics and binding thermodynamics.
Duties and Responsibilities:
Qualifications
About the company
Vanderbilt University is a private research university in Nashville, Tennessee.
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